Eastleigh will be playing against Gosport Borough in Blue Square Bet South next season.
Alex Pike's side won the Evostik Southern Premier play-off final 5-4 on penalties after drawing 2-2 with Gosport Borough.
Gosport led 2-0 through Luke King and an Adam Wilde free-kick before Hemel Hempstead hit back, forcing extra time with an injury-time equaliser.
King, Wilde, Ellis Martin, Justin Bennett and Sammy Igoe all netted from the spot to ensure promotion to the sixth tier of English football for the first time in Gosport's 69-year history.
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